Figure 6.

hGM-CSF effects on Lin⁻Sca-1⁺c-kit⁺ BM cells. Lin⁻Sca-1⁺c-kit⁺ cells were purified from BM of the chimeric receptor transgenic mice by FACS sorting. One hundred Lin⁻Sca-1⁺c-kit⁺ cells were incubated with hGM-CSF and SCF (▪), IL-6 and SCF (□), or IL-11 and SCF (░⃞) in serum-free suspension cultures. The starting 100 Lin⁻Sca-1⁺c-kit⁺ cells contained 50.7 CFU-Mix, 33.3 CFU-GM, and 13.3 CFU-Meg. On day 8 of incubation, total cell number, percent of Sca-1⁺c-kit⁺ cells, and number of CFU-Mix were examined. The y-axis represents expansion rates of Sca-1⁺c-kit⁺ cells (A) and CFU-Mix (B). Assays were done in triplicate cultures and repeated in two independent experiments.