Table 1.
Colony formation from BM cells of the chimeric receptor transgenic mice
| Cytokines | E | GM | Mix | B | Mast | |
|---|---|---|---|---|---|---|
| Mice | ||||||
| Transgenic | hGM-CSF + Epo | 94.7 ± 11.4 | 36.7 ± 0.6 | 6.3 ± 1.5 | 4.0 ± 1.7 | 0.0 ± 0.0 |
| IL-3 + Epo | 90.7 ± 7.6 | 73.3 ± 1.5 | 4.0 ± 1.7 | 2.0 ± 0.0 | 7.0 ± 1.0 | |
| Epo | 110.0 ± 13.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| Normal | hGM-CSF + Epo | 102.0 ± 7.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| IL-3 + Epo | 92.7 ± 9.2 | 76.0 ± 3.6 | 4.0 ± 0.0 | 1.3 ± 1.5 | 4.3 ± 1.5 | |
| Epo | 100.7 ± 21.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| Cytokines | ||||||
| hGM-CSF + SCF + Epo | 26.3 ± 4.9 | 6.7 ± 2.1 | ||||
| IL-6 + SCF + Epo | 22.3 ± 4.2 | 5.0 ± 1.7 | ||||
| IL-11 + SCF + Epo | 25.0 ± 1.0 | 4.0 ± 1.0 | ||||
| hGM-CSF + Epo | 4.3 ± 0.6 | 2.0 ± 2.0 | ||||
| IL-6 + Epo | 3.0 ± 2.0 | 0.3 ± 0.6 | ||||
| IL-11 + Epo | 1.3 ± 1.5 | 0.0 ± 0.0 | ||||
| SCF + Epo | 1.7 ± 1.1 | 0.0 ± 0.0 | ||||
| Epo | 0.0 ± 0.0 | 0.0 ± 0.0 |
For mice, 20,000 BM cells from the chimeric receptor transgenic mice or from their normal littermates were cultured in the presence of the designated cytokine(s). E, erythroid colonies; B, erythroid bursts. For cytokines, 2000 Lin− BM cells from 5-FU-treated chimeric receptor transgenic mice were cultured in the presence of the designated cytokine(s). The number of colonies indicates mean ± SD of triplicate cultures.