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(a–e) Immunogold labeling of myosin-V in B16 cells, embedded in LX-112 resin, with affinity-purified anti-myosin-V tail at 160 μg/ml and a secondary antibody conjugated to 10-nm gold particles. (f) Control labeling with the primary antibody preadsorbed with excess of purified brain myosin-V. Melanosomes at stages I, II, III, and IV, mitochondria (MI), small vesicles (arrowheads), Golgi (G), and endoplasmic reticulum (ER) are indicated. Bars, 0.3 μm.
