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Immunogold labeling of myosin-V in B16 cells embedded in LR-White resin, with affinity-purified anti-myosin-V tail at 160 μg/ml and 10-nm (a–c, e, h, k, and l) or 18-nm (d, f, g, i, and j) gold particles conjugated secondary antibody. Several examples of labeling on or close to melanosomes at stages I, II, III, and IV are shown (arrowheads). Note that labeling is also seen on filaments (a, b, and e) or vesicular structures (c and i) associated with melanosomes. Bar, 0.24 μm.
