Figure 4.

AP-1 subunit gene deletions enhance the α-factor maturation defect in chc1-ts cells. Strains aps1Δ chc1-ts (GPY719; lanes 1 and 10), apm1Δ chc1-ts (GPY 1423; lanes 2 and 11), apl2Δ chc1-ts (GPY 906; lanes 3 and 12), apl4Δ chc1-ts (GPY 1352; lanes 4 and 13), apl2Δ apl4Δ chc1-ts (GPY 1353; lanes 5 and 14), apl2Δ apl4Δ apm1Δ aps1Δ chc1-ts (GPY 1627–2C; lanes 6 and 15), apl2Δ apl4Δ apm1Δ aps1Δ (GPY 1599-23D; lanes 7 and 16), wild type (GPY 1100; lanes 8 and 17), and chc1-ts (GPY 418; lanes 9 and 18) were grown overnight at 24°C and then shifted to 24 or 30°C for 2 h. Cells were metabolically labeled with [³⁵S]methionine and cysteine for 45 min at 24 or 30°C. α-Factor was immunoprecipitated from the culture supernatant and subjected to SDS-PAGE and autoradiography. −, deletion of a gene; + or blank, presence of a gene; ts, presence of chc1-ts.