Table 1. Efficiency of co-transfection of hLF BAC and pCEIN by microinjection.

Manipulated cells	GFP and Neor positive colonies*		hLF BAC integration colonies†
	Number	Efficiency(%)	Number	Efficiency(%)
2050	21	1.02	2	9.76×10−2
2300	17	0.74	2	8.70×10−2
1900	18	0.95	3	15.79×10−2

^*After approximately 20 days, the positive colonies (expressing GFP and Neo^r) were screened by G418 and confirmed by the expression of GFP.

^†After the GFP and Neo^r positive colonies were selected and expanded, DNA extraction was performed using some cells from each colony, and the integrated cells were determined by PCR using primer P1, P2 and P3.