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. 2008 Oct 20;3(10):e3457. doi: 10.1371/journal.pone.0003457

Figure 3. Assay validation.

Figure 3

(A) Detrended luminescence traces of wells corresponding to either Bmal1 (left, n = 4), Cry1 (right, n = 4) or scrambled control siRNA (gray traces, both panels, n = 16). Relative mRNA (±s.d.) values were determined by Q-PCR (Inlets). (B) Detrended Luminescence traces of wells transfected with different siRNAs specific for the indicated circadian oscillator components (n = 4) or scrambled unspecific siRNA control wells (n = 16). (C) Amplitude and period length plots of the results obtained with siRNAs depicted in (a) and (b) (n = 4, s.e.m). Multiples of s.e.m. of the control siRNA (n = 16) are indicated in gray boxes. Arrhythmic wells are displayed as open circles.