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. 1998 Dec;9(12):3445–3453. doi: 10.1091/mbc.9.12.3445

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Copyright © 1998, The American Society for Cell Biology

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Immunoblot analysis of SCD degradation. Nitrocellulose blots were probed with rabbit antiserum to SCD (lanes 1–6) and rabbit antiserum to a C-terminal peptide of SCD (lanes 7–8). The samples analyzed were the DOC/TX100-soluble fraction of DSIMs before (lanes 1 and 7) and after (lanes 2 and 8) incubation at 37°C and the DOC/TX100-soluble fraction of CRLMs unsupplemented (lanes 3 and 4) and supplemented with 2 μg purified SCD (lanes 5 and 6) before (lanes 3 and 5) and after (lanes 4 and 6) incubation at 37°C.