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Isolation of the 20-kDa fragment of SCD. Solubilized DSIMs were incubated to generate the 20-kDa fragment. The preparation was passed over a DEAE-cellulose column. (A) Coomassie blue–stained SDS-PAGE gel of the DEAE flow-through fraction. (B) Immunoblot analysis of the DEAE flow-through fraction using antibody against the C terminus of SCD.
