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. 2008 Oct 20;3(10):e3450. doi: 10.1371/journal.pone.0003450

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Lizano et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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cDNA generated from RNA derived from wt Alab49 (A) or Alab49 rofA::aad9 (B) was used as a template for PCR amplification; amplicons were subject to agarose gel electrophoresis and ethidium bromide staining. All reactions are paired with reverse primer CPA-R in combination with UP-F1 (lane 1), UP-F2 (lane 2), UP-F3 (lane 3), UP-F4 (lane 4) and CPA-F (lane 5). Positions for primers UP-F1 through F4 are also depicted in Figure 1C. Molecular size markers are indicated.