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. 2008 Oct 20;3(10):e3450. doi: 10.1371/journal.pone.0003450

Figure 6. Decrease in pilus gene transcript levels in rofA::aad9 ΔmsmR.

Figure 6

qRT-PCR data showing transcript abundance for rofA::aad9 and rofA::aad9 ΔmsmR relative to wt Alab49, for cpa (panel A) and fctA (panel B). RNA was prepared from three bacterial cultures - wt, rofA::aad9 and rofA::aad9 ΔmsmR - grown to the mid-logarithmic phase in three separate experiments; two independent cDNA preparations were generated from each RNA preparation. Matched pairs of strains from the six different cDNA preparations are plotted. Statistical significance is measured for transcript ratios of rofA::aad9 versus rofA::aad9 ΔmsmR by the paired t-test (2-tailed).