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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Biol Chem. 2008 Sep;389(9):1235–1238. doi: 10.1515/BC.2008.XXX

Figure 2.

Figure 2

SDS-PAGE of azidomethaemoglobin (A), methaemoglobin (B) and apohaemoglobin (C) during incubation with Kgp. (D) Control apohaemoglobin minus Kgp. Incubation conditions as in Figure 1. Haem-free apohaemoglobin was prepared by the acid-acetone method of Ascoli et al. (1981). Enzyme and substrate concentrations were 0.4 and 4 µM, respectively. SDS-PAGE was carried out on 15% acrylamide gels after sample solubilisation at 100°C for 5 min under reducing conditions as previously described (Smalley et al., 2007). And, 5 µg of protein was loaded per track.