Figure 7.

Secretory precursor interaction with the receptor component Sec72p in sec61-11 membranes can be partly restored by adding wild-type Sec61p from an sec71 or sec72 deletion strain. Detergent (octylglucoside) extracts of the indicated wild-type (Wt) or mutant membranes were prepared and mixed in equal amounts before reconstitution of proteoliposomes by dialysis as described in MATERIALS AND METHODS. Binding of wild-type and m3-mutant ppαf precursor to Sec72p in the proteoliposomes reconstituted from 200 μg of solubilized membranes was assayed as in Figure 4. Each bar is the average of two experiments.