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. Author manuscript; available in PMC: 2008 Oct 9.
Published in final edited form as: Chem Res Toxicol. 2007 Feb 1;20(2):316–321. doi: 10.1021/tx600340c

Figure 2.

Figure 2

Analysis of styrene mercapturic acids in a competitive ELISA using antiserum #1043 (A) and #1044 (B) raised against immunogen II. Ninety-six-well microtiter plates were coated with bovine albumin–styrene oxide adducts (20 μg/mL) and blocked with 5% nonfat milk. The resulting plates were incubated with corresponding antisera pretreated with serial dilutions of competitor styrene mercapturic acids (0.1 to 10−11 mM), followed by the sequential addition of antibody and substrate. The absorbance at dual wavelengths (450–650 nm) was read. The values are expressed as the mean ± SD from triplicate assays.