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. 2008 Oct 9;105(42):16368–16373. doi: 10.1073/pnas.0806222105

Fig. 6.

Fig. 6.

Cleavage of procaspase-2 and Bid are PIDD-dependent. (A) Effect of siRNA against PIDD in the cytosol 8 h after tGCI. Western blot analysis showed that PIDD-FL and PIDD-CC expression significantly decreased in the 5-μg PIDD-siRNA-treated animals 8 h after tGCI (n = 4; *, P < 0.05). Cleavage of procaspase-2 was significantly inhibited in the PIDD-siRNA-treated animals (n = 4; *, P < 0.05; †, P < 0.01; ‡, P < 0.005). There was no significant difference in RAIDD expression in any groups. β-actin was used as an internal control. tGCI, nontreated; cont, control-siRNA-treated; OD, optical density. (B) Effect of siRNA against PIDD in mitochondria 8 h after tGCI. Western blot analysis demonstrated that mitochondrial tBid expression significantly decreased in the 5-μg PIDD-siRNA-treated animals 8 h after tGCI (n = 4; ‡, P < 0.005). Mitochondrial Bax and Bcl-XL showed no significant difference among all groups. Cytochrome oxidase subunit IV (COX IV) was used as an internal control.