Figure 3.

A Single Dose of Active Virus Significantly Suppresses Hepatic CYP3A2 mRNA in Male Sprague Dawley Rats. Mean relative intensities of CYP3A2 mRNA and representative agarose gels of PCR products 0.25 (A), 1 (B), 4 (C), and 14 (D) days after treatment with wild type adenovirus 5 (WT), first generation recombinant adenovirus expressing E. coli beta-galactosidase (AdlacZ), PEGylated AdlacZ, (PEGAd), helper-dependent virus expressing beta-galactosidase (HDAd), or inactivated AdlacZ (UVAd). mRNA levels are reported as band densities of gene-specific RT-PCR products with respect to the density of products from an internal control (18S rRNA) in arbitrary units. In all panels, animals dosed with phosphate buffered saline served as controls (Vehicle). Values are presented as the mean ± standard error of 4 animals/treatment/timepoint. Statistical significance was determined between individual treatment groups and vehicle controls by one-way analysis of variance with a Bonferroni/Dunn post-hoc analysis. *p ≤ 0.05, **p ≤ 0.01.