Fig. 6.
Expression at the cell surface and functional assay of the receptors containing the GABAB2 subunit bearing the double mutation of R575 and D688. A. The cell surface expression of the receptors was analyzed using an ELISA method on intact cells as described in figure 3. The data are the means ± sem of at least three independent experiments performed in triplicates. B. Functional responses of mutated receptors. The ligand-induced activity of the mutated receptors was measured as described in figure 3. The data are the means ± sem of at least three independent experiments performed in triplicates.