Table 2. Evolution of clinical parameters of vervet monkeys that were infected with T. b. rhodesiense cloned strains through tsetse (Glossina pallidipes) transmission.
| Vervet monkey clinical data | T.b.r. KETRI 3741 | T.b.r. KETRI 3801 | T.b.r. KETRI 3804 | T.b.r. KETRI 3928 | ||||||
| a476F | a515M | a536M | a523F | a579M | a556F | a574M | a554F | a555M | ||
| Appearance of trypanosome chancre: dpi when first seen | NO | 4 | 4 | NO | NO | 4 | 4 | NO | 4 | |
| Pre-patent period: dpi | 4 | 4 | 4 | 4 | 8 | 4 | 4 | 10 | 5 | |
| Enlargement of superficial lymph nodes: dpi when first seen | 8 | 8 | 12 | 8 | 8 | 8 | 8 | 12 | 4 | |
| Splenomegaly | dpi when first seen | 12 | 8 | 12 | 8 | 12 | 8 | 32 | 16 | 32 |
| Size relative to pre-infection | ×1.5 | ×1.5 | ×2 | ×1.5 | ×1.5 | ×1.5 | ×1.5 | ×2 | ×1.5 | |
| Muscle tremors: dpi when first seen | NO | NO | NO | NO | 12 | NO | NO | NO | NO | |
| Cerebrospinal Fluid parasitosis | First detection: dpi | 16 | 24 | 16 | 16 | 8 | 16 | 24 | 40 | 16 |
| No. of trypanosomes in CSF during the entire infection: median (range) | 1 (0–123) | 15.5 (0–26) | 2 (0–475) | 1 (0–1) | 1 (0–31) | 4 (0–31) | 1.5 (0–1620) | 1 (0–10) | 3.5 (0–363) | |
| CSF white cell (WC) changes | Pre-infection: median (range) | 1 (0–8) | 6 (3–9) | 7 (7–8) | 0 | 1 (1–3) | 4 (1–6) | 1 (0–1) | 3 (0–7) | 1 (1) |
| During infection: median (range) | 16 (4–91) | 11 (2–45) | 14 (4–20) | 3 (0–7) | 9 (2–14) | 8 (0–39) | 7.5 (0–69) | 4 (0–20) | 17 (3–46) | |
| Ataxia/incoordination: dpi when first seen | NO | NO | 28 | 16 | 35 | NO | NO | 115 | NO | |
| Oedema of eyelids, vulva or scrotum: dpi when first seen | 84 | NO | NO | NO | 37 | 76 | NO | NO | NO | |
| Abnormal posture | NO | NO | NO | O | O | NO | NO | NO | NO | |
| Lethargy/reduced activity at extremis | O | NO | O | O | O | O | O | O | O | |
| Somnolence at extremis | O | NO | O | O | O | NO | NO | O | NO | |
| Inability or extreme reluctance to perch: dpi when first seen | 104 | 46 | 34 | NO | NO | NO | NO | NO | 88 | |
| Duration of disease up to euthanasia at extremis (dpi) | 104 | 46 | 34 | 21 | 41 | 84 | 68 | 120 | 88 | |
| Reduced appetite | Proportion of days in which feed intake was 50% or below of daily ration (pre-infection) | 3/14 (21.4%) | 0/14 (0%) | 0/14 (0%) | 1/14 (7.1%) | 0/14 (0%) | 0/14 (0%) | 0/14 (0%) | 0/14 (0%) | 0/14 (0%) |
| Proportion of days in which feed intake was 50% or less of daily ration (after infection) | 13/104 (12.5%) | 5/46 (10.9%) | 12/34 (35.3%) | 11/21 (52.4%) | 2/41 (4.9%) | 0/84 (0%) | 1/68 (1.5%) | 0/120 (0%) | 1/120 (0.8%) | |
| Pre-infection weight (kg) | 2.7 | 3.4 | 4.1 | 2.7 | 4.3 | 2.9 | 4.0 | 3.6 | 5.2 | |
| %weight loss at 32 dpi | 3.8 | 10.3 | 32.1 | NDA | 23.3 | 7.0 | 13.8 | 15.5 | 12.6 | |
Key: dpi = days post infection; T.b.r. = Trypanosoma brucei rhodesiense; a = Laboratory number of monkey; F = Female; M = Male; O = observed; NO = Not observed; − = decreased; + = increased.