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uvsB and uvsD mutants fail to arrest in response to DNA damage in G2. The four indicated nimA5 strains were arrested in G2 at 42°C before downshift to 32°C to allow synchronous release into mitosis. Cells were released with or without MMS-induced DNA damage as indicated, and the chromosome mitotic index was determined in >200 cells per time point after staining DNA of fixed cells with DAPI.
