Figure 8.
nimXcdc2AF + bimE7APC1 double mutant cells display polyploid nuclei. DAPI staining of cells showing representative nuclear morphologies observed in the double mutant germlings are shown. (A) 12-h-old germling with a highly stretched large nucleus (17% of cells). (B) Germling showing incomplete DNA segregation indicated by the arrows (50% of cells). (C) Part of a hyphae showing large abnormal nuclei (33% of cells). (D) Double mutant spores 12 h after germination at 42°C, showing the typical mitotic block phenotype of the bimE7 mutation with highly condensed DNA. Arrows indicate stretched nuclei. Bar, 5 μm.