Figure 9.

Loss of NIMX^cdc2 and NIMA kinase activities and proteolysis of NIME^cyclinB in nimX^cdc2AF mutant cells after addition of 6 mM HU. HU was added to log phase cultures of either a wild-type or the nimX^cdc2AF mutant strain to the final concentration of 10 mM, and samples were collected at the time intervals indicated. NIMX^cdc2 (A) and NIMA (B) kinase activities were assayed using histone H1 or β-caesin as substrate. NIME^{cyclin B} (C) and NIMX^cdc2 (D) protein levels were determined by Western blotting.