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. 2008 Oct;9(10):823–828. doi: 10.1631/jzus.B0860006

Table 1.

Sequences of the primers used for this research

Primer name Nucleotide sequence (5′ to 3′) Tm (°C)
hph1 TTCGATGTAGGAGGGCGTGGA 58
hph2 CGCGTCTGCTGCTCCATACAAG 58
LB1 GGGTTCCTATAGGGTTTCGCTCATG 64
LB2 CATGTGTTGAGCATATAAGAAACCCT 60
LB3 GAATTAATTCGGCGTTAATTCAGT 56
RB1 GGCACTGGCCGTCGTTTTACAAC 64
RB2 AACGTCGTGACTGGGAAAACCCT 62
RB3 CCCTTCCCAACAGTTGCGCA 61
AD1 WGTGNAGWANCANAGA 45
AD2 NGTCGASWGANAWGAA 47
AD3 NTCGASTWTSGWGTT 45

Nucleotide sequences of hph1 and hph2 from Irie et al.(2001) and nucleotide sequences of others from Rooney et al.(2001) and Sambrock et al.(1989); T m: melting temperature