Table 1.
Primer name | Nucleotide sequence (5′ to 3′) | Tm (°C) |
hph1 | TTCGATGTAGGAGGGCGTGGA | 58 |
hph2 | CGCGTCTGCTGCTCCATACAAG | 58 |
LB1 | GGGTTCCTATAGGGTTTCGCTCATG | 64 |
LB2 | CATGTGTTGAGCATATAAGAAACCCT | 60 |
LB3 | GAATTAATTCGGCGTTAATTCAGT | 56 |
RB1 | GGCACTGGCCGTCGTTTTACAAC | 64 |
RB2 | AACGTCGTGACTGGGAAAACCCT | 62 |
RB3 | CCCTTCCCAACAGTTGCGCA | 61 |
AD1 | WGTGNAGWANCANAGA | 45 |
AD2 | NGTCGASWGANAWGAA | 47 |
AD3 | NTCGASTWTSGWGTT | 45 |