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. 2008 Oct 22;3(10):e3482. doi: 10.1371/journal.pone.0003482

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Schneider et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HCMV-infected (MOI 50) pDCs (pDC+HCMV) and pDCs incubated with UV-inactivated virus (pDC+HCMV-UV) were irradiated (30 Gray) at day five post infection. 5×10⁴–5×10² tpDCs (A) or bpDCs (B) were added as stimulator cells to 10⁵ allogeneic purified CD4⁺ T cells (responder cells). As a control, mock-infected tpDCs or bpDCs (pDC mock) were included in each experiment. To measure T cell proliferation, cells were labelled with 1µCi ³H-thymidine per well at day five after coculture. The incorporation of ³H-thymidine (cpm) was measured for 18 h.