(A–C): Serial sections from a representative AD case were incubated with immunized serum (A and B) or preimmune serum (C). Specific staining was only observed following application of immunized serum. (D–F): Serial AD sections were immunolabeled with purified TDPccp (1:200) and labeling of Hirano bodies (B), dystrophic neurites (arrowhead, D), plaques (arrow, D), or reactive astrocytes (E) was observed. In contrast, staining with purified TDPccp was prevented after preadsorption with free peptide (F). All scale bars represent 10 µm, except for Panel F, which represents 20 µm.