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. 2008 Jul 14;52(10):3725–3736. doi: 10.1128/AAC.00163-08

FIG. 2.

FIG. 2.

(A) Schematic representation of the blaKPC-2-containing fragment (GenBank accession numbers DQ989639 and DQ989640) obtained by several amplifications with the primers IS1 S, IS1 AS, Tase 4, and Tase 1 (Table 1). The transcriptional orientations of the two flanking ORFs, Orf1 and Orf2, are indicated by arrows. The broken line upstream from blaKPC-2 indicates a lacking stretch of nucleotides compared with previously reported sequences surrounding blaKPC-2. The putative promoter found 41 bp upstream from the deletion is indicated (−35 and −10 sites). (B) Schematic representation of the BamHI segment containing the blaKLUC-2 gene (GenBank accession number EF057432). The truncated transposase gene tnpA of the ISEcp1 element (tnpAΔ) is found upstream from blaKLUC-2. The blaKLUC-2 promoter region is indicated by an open circle. The black box represents the right inverted repeat of ISEcp1 (IR-R). (C) Schematic representation of the BamHI fragment containing the blaTEM-1 gene and the genes coding for the resolvase (tnpR) and a part of the transposase (tnpAΔ), respectively (GenBank accession number AB187515).