TABLE 1.
Nucleotide sequences of primers
| Primer | Sequencea | Gene | Annealing temp (°C) | Reference or source |
|---|---|---|---|---|
| SHV rev | 5′-GCGTTGCCAGTGCTCGATCAG-3′ | blaSHV | 62 | 3 |
| SHV bis | 5′-ATGCGTTATATTCGCCTGTGTATT-3′ | |||
| TEM H | 5′-TGAGATCGAAGGGCCGTT-3′ | blaTEM | 52 | 53 |
| TEM rev | 5′-GGTCTGACAGTTACCAAT-3′ | |||
| SmeA | 5′-CGGTCCTGA GGGGATGAC-3′ | blaSme-1 | 53 | 38 |
| SmeB | 5′-CGTGATGCTTCCGCAATA-3′ | |||
| KPC 1S | 5′-ATGTCACTGTATCGCCGT-3′ | blaKPC-1 | 48 | 61 |
| KPC 1AS | 5′-CCTTACTGCCCGTTGACG-3′ | |||
| KPCINT S | 5′-ATGACTATCCCGTCGGC-3′ | blaKPC-1 | 52 | 61 |
| KPCINT AS | 5′-CATCGCGTACACACCGATGGA-3′ | |||
| KLUC-1 S | 5′-ATGGTTAAAAAATCATTA-3′ | blaKLUC-1 | 47 | 15 |
| KLUC-1 AS | 5′-CTATAATCCCTCAGTGAC-3′ | |||
| CTX-M1A | 5′-CTTCCAGAATAAGGAATC-3′ | blaCTX-M | 48 | 6 |
| CTX-M1B | 5′-CCGTTTCCGCTATTACAA-3′ | |||
| CTX-MA | 5′-CGCTTTGCGATGTGCAG-3′ | |||
| ISEcpI 1A | 5′-AATCTAACATCAAATGCA-3′ | 50 | 45 | |
| ISEcpI 1B | 5′-TTTTGCTGCAAGAAATACATA-3′ | |||
| IS1 S | 5′-ATCGCCTTGGGTTACAAG-3′ | 49 | 62 | |
| IS1 AS | 5′-CTTGTCCTTCAAGCGGTA-3′ | |||
| KPC-EcoRI | 5′-CCGGAATTCTTACTGCCCGTTGACGCCCAA-3′ | blaKPC-1 | 60 | Present study |
| KPC-NdeI | 5′-GAATTCCATATGTCACTGTATCGCCGTCTAGTT-3′ | |||
| Tase 4 | 5′-ATGGCTGCCAGTTACCTT-3′ | 54 | 59 | |
| Tase 1 | 5′-CCTTGGCATCTTCACGTCCCT-3′ | |||
| KPdelS | 5′-CGAGGGATTGGGCTAAGAATTCGAGC-3′ | 54 | Present study | |
| KPdelAS | 5′-GCTCGAATTCTTAGCCCAATCCCTCG-3′ |
a
Where present, underlining indicates the restriction site for the endonuclease included in the primer designation.