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Disk diffusion assay demonstrating that HA but not HA-P has bioactivity against the efflux pump-deficient ΔtolC E. coli strain. Filter paper disks with 30 or 60 μg of both HA and HA-P were placed on LB agar plate seeded with ΔtolC E. coli and incubated overnight at 37°C.
