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Reversed-phase HPLC analyses and radiolabel detection of wild-type and Δhyg21 culture broths grown in the presence of [2-3H]myo-inositol. The production of HA was significantly decreased in the mutant. Radiolabeled analogs of HA (see Fig. 1 for structures) are present in fermentation broths of both the wild-type and the Δhyg21 strains.
