TABLE 5.
Results of sequencing of the ampD genes from a collection of 10 ceftazidime-resistant, AmpC-hyperproducing clinical strains
| Strain | ampD mutationa | Polymorphismb
|
|
|---|---|---|---|
| AmpDh2 | AmpDh3 | ||
| BCL2A8 | 1-bp insertion (C) in nt 53 | None | Ala219Thr |
| MCV1C6 | None | None | Ala219Thr |
| FMC1H6 | None | None | Ala208Val |
| JSG2A1 | 1-bp insertion (C) in nt 481 | None | Ala219Thr |
| VFF2E2 | None | None | Ala208Val |
| JCM2C2 | 1-bp deletion (C) in nt 449 | Val40Ile | None |
| MSF2F5 | None | None | None |
| MSC2A9 | 1-bp insertion (G) in nt 297 | None | Ala219Thr |
| MQB1C5 | C→T (stop codon) at nt 463 | None | Ala219Thr |
| OFC2I4 | Complete deletion of the ampDE region | None | None |
a
Nucleotide (nt) numbers correspond to the PAO1 ampD sequence. ampD mutations were obtained from reference 16. None of the ampD mutations were present in the isogenic ceftazidime-susceptible isolates (16).
b
Amino acid numbers correspond to the PAO1 AmpDh2 or AmpDh3 sequence. All the polymorphisms detected were also present in the isogenic ceftazidime-susceptible isolates.