TABLE 1.

Measurement of apoptosis by TUNEL technique^a

Cells	Treatment	% TUNEL assay-positive cells
L. infantum wild type	Control	0
L. infantum wild type	40 μM edelfosine, 24 h	42.5 ± 4.7
L. infantum pX63	Control	0
L. infantum pX63	40 μM edelfosine, 24 h	47.4 ± 5.2
L. infantum Bcl-XL	Control	0
L. infantum Bcl-XL	40 μM edelfosine, 24 h	26.7 ± 3.1
L. infantum Hrk	Control	0
L. infantum Hrk	40 μM edelfosine, 24 h	92.5 ± 8.3

^aApoptosis was measured by the TUNEL technique with untreated control cells and cells treated with 40 μM edelfosine for 24 h. Wild-type parasites and parasites transfected with the empty expression vector pX63 (L. infantum pX63), pX63-bcl-X_L (L. infantum Bcl-X_L), or pX63-hrk (L. infantum Hrk) were used. The data are shown as the means ± standard deviations (n = 3).