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Anti-ubiquitin Western blot. (A) Cells were taken at the times in development indicated, lysed in PAGE-SDS gel buffer, sized on a 10% gel, blotted to Immobilon-P membrane (Millipore), and probed with a commercial antiubiquitin antibody (Sigma, St. Louis, MO). The position of the size markers are shown. Band a, ca. ∼73 kDa; band b, ca. ∼59 kDa; band c, ca. ∼51 kDa. (B) Enlargement of the section of the blot containing bands a–c.
