FIG. 1.

Diagram and sequences of the huCYP51 replacement cassette. The upstream and downstream flanking regions of S. cerevisiae CYP51 are shown in uppercase. The huCYP51 sequence with S. cerevisiae codon bias (codon adaptation index = 0.571 [25]) and conflicting restriction enzyme sites removed is shown in lowercase. NotI sites for excision from the pUC57 vector are shown underlined. BglII and SpeI sites for insertion of the URA3 marker are shown in boldface. Transcription initiation (AAA) and transcription termination (polyadenylation, TAAATAAA) sequences are in boldface and underlined. The sequence between the stop codon (taa) and the polyadenylation site contains the sequence for a histidine tag not utilized in this study.