FIG. 2.

(A) Transcription levels of native ERG11 and huCYP51. RT-PCR was performed, mean starting quantities were determined, and data were normalized to the values for yeast actin. Error bars show SEs for different quantities. (B) Western blot analysis of huCYP51p localization from cytosolic fractions (lanes 1 and 3) and microsomal fractions (lanes 2 and 4). Molecular mass markers (M) are shown in kilodaltons. An arrow indicates the band corresponding to huCYP51p (56.8 kDa), present in a microsomal fraction from the BY4741:huCYP51 strain.