TABLE 4.
Proteins downregulated in planktonic and biofilm cells of Salmonella serovar Enteritidis following continuous sublethal (1-μg ml−1) BC exposure for 144 h
| Class or function(s) | Spot no. | Gene | Protein description | Ma (kDa) | pIb | Fold changec for:
|
|
|---|---|---|---|---|---|---|---|
| Planktonic cells | Biofilm cells | ||||||
| Degradation (carbon compounds) | 6 | garR | Tartronate semialdehyde reductase | 30.73 | 5.59 | 0.58 | 0.09 |
| Energy metabolism (glycolysis) | 7 | gpmA | Phosphoglycerate mutase 1 | 28.36 | 5.78 | 0.46 | 0.02 |
| Ribosomal protein synthesis and modification | 8 | rpsA | 30S ribosomal subunit protein S1 | 61.25 | 4.89 | 0.38 | 0.08 |
| Protein translation and modification | 9 | tufA | Translation elongation factor (EF-Tu.A) | 43.40 | 5.30 | 0.27 | 0.25 |
| Cell envelope (LPS) | 10 | rfbH | LPS biosynthesis protein | 48.10 | 5.27 | 0.66 | 0.06 |
| Cell processes (binding proteins, other) | 11 | oppA | Oligopeptide-binding protein complexed with Kvk, chain A | 58.81 | 5.85 | 0.54 | 0.09 |
| Cell processes (chaperones) | 12 | dnaK | Chaperone protein Hsp70 | 69.13 | 4.83 | 0.65 | 0.17 |
a
M, molecular mass.
b
pI, isoelectric point. Values are theoretical values obtained from the Swiss-Prot or PUMA2 database.
c
Ratio of protein expression in adapted cells to the protein expression in nonadapted (control) cells.