FIG. 6.
Itraconazole binding by C. albicans CYP56. Itraconazole was progressively titrated against Ni2+-NTA agarose-purified C. albicans CYP56 at 2 μM. A characteristic type II azole-binding spectrum was observed when spectrophotometric determinations were made between 350 and 500 nm after each addition of itraconazole. (Inset) A plot of ΔA430-412 against the itraconazole concentration was constructed to determine the kd for itraconazole by using nonlinear regression (Levenberg-Marquardt algorithm) of the Hill equation.