TABLE 2.
Primers used in this study for the deletion of CYP56 in C. albicans
| Primer | Sequencea | Reference |
|---|---|---|
| DITFOR | 5′-TTGGCCAAGGAATATTTCTG-3′ | This study |
| DITREV | 5′-TGTGCTTTGATGTACCTGTG-3′ | This study |
| CYP56F | 5′-CATCGCCATATGGCTCAACTATTGAAATAT-3′ | This study |
| CYP56R | 5′-ACTGCTGCATGCCTAGTGATGGTGATGGTGATG GTGATGGTGATGGGAAACTGTAAGCTTTTC-3′ | This study |
| DIT2F | 5′-ATGCGAGCTCAGTTTGTTACCAATATGCAC-3′ | This study |
| DIT2R | 5′-ATCCGGATCCGCCATACTATGCCATTAAGG-3′ | This study |
| CYP56KF | 5′-GGCCGGTACCAGTTTGTTACCAATATGCAC-3′ | This study |
| CYP56XR | 5′-GGCCCTCGAGAATGGTGGGAAAACTATCTC-3′ | This study |
| DITSIIF | 5′-GCCGCCGCGGTTCTAGGTGAGTATAAAGTG-3′ | This study |
| DITSIR | 5′-GGCCGAGCTCGCCATACTATGCCATTAAGG-3′ | This study |
| SAT1 | 5′-GCCCGACGTCGCACTCGAGCGTCAAAACTAG-3′ | 12 |
| SAT2 | 5′-CTAGTGATTTCTGCAGGACCACCTTTG-3′ | 12 |
| MAL1 | 5′-CATGCAAGCCAGGATCCAATAATGATTGG-3′ | 12 |
| MAL2 | 5′-GTTCACTCATTGTCGACGATTATTAGTTAAACC-3′ | 12 |
| FLP1 | 5′-TTCCGTTATGTGTAATCATCC-3′ | 12 |
| FLP2 | 5′-CGCTGAGTTTCGATATTGTC-3′ | 12 |
| FLP3 | 5′-GTATATGTGCCTACTAACGC-3′ | 12 |
| RT300 | 5′-GTACTTGACATGCCATACTATGC-3′ | This study |
| RT768 | 5′-AGTTTCGATAAAGAAAACAAGA-3′ | This study |
| TEM1R | 5′-TGGTTATTCTGATCCTGTTG-3′ | This study |
| ACT10 | 5′-GCGGAATTCAGAGTCGACATT-3′ | This study |
a
Underlined sequences represent the following restriction sites: SacI for DIT2F, BamHI for DIT2R, KpnI for CYP56KF, XhoI for CYP56XR, SacII for DITSIIF, and SalI for DITSIR.