FIG. 1.

RAP-PCR patterns of total RNA from nonadapted and acid-adapted S. macedonicus cells. The profiles were obtained from nonadapted control cells (lane a), autoacidified cells (lane b), transiently adapted cells (lane c), acid-habituated cells (lane d), and stationary-phase cells (lane e). Bands exhibiting higher abundance than the control bands were excised from the gel, reamplified, cloned, and sequenced. cDNA fragments that were found to correspond to protein-encoding genes (B1 to B5) and internal regions of either 16S or 23S rRNAs are indicated by arrows and arrowheads, respectively. The dendrogram (% similarity) was inferred by the Dice similarity coefficient and the UPGMA clustering algorithm to identify pattern similarities among the RAP-PCR fingerprints.