TABLE 2.
LH-PCR fragment length database of 13 strains isolated from cheese samples belonging to different RAPD-PCR clusters and 6 type strains
Strain | RAPD-PCR cluster or branch | No. of isolatesa | Origin | Closest relative or type strainb | Accession no.c | % Match | Fragment size (bp)d |
---|---|---|---|---|---|---|---|
750 | 23 | 1 | Curd, 12 h | Kocuria kristinae | AF375912 | 100 | 310 |
714 | 1 | 2 | Milk | Lactobacillus delbrueckii subsp. lactis | AB289095 | 99 | 330 (284) |
779 | 2 | 2 | Curd, 48 h | Lactobacillus helveticus | AB008210 | 100 | 334 |
776 | 22 | 9 | Curd, 6 h | Lactobacillus helveticus | AB008210 | 98 | 334 |
1056 | 34 | 2 | Cheese, 6 mo | Lactobacillus casei | AJ558112 | 100 | 335 |
1247 | 35 | 1 | Curd, 12 h | Lactobacillus casei | AB008205 | 100 | 335 |
830 | 10 | 85 | Salted cheese | Lactobacillus rhamnosus | EU184020 | 100 | 336 (290) |
1489 | 28 | 1 | Cheese, 20 mo | Lactobacillus rhamnosus | EF533991 | 99 | 336 (290) |
710 | 30 | 1 | Curd, 48 h | Lactobacillus plantarum subsp. plantarum | EF577047 | 99 | 337 (290, 308) |
1026 | 3 | 2 | Cheese, 6 mo | Lactobacillus fermentum | AF477498 | 99 | 342, 344 |
730 | 31 | 7 | Curd, 6 h | Lactobacillus fermentum | EU221276 | 99 | 342, 344 |
1466 | 12 | 1 | Cheese, 20 mo | Pediococcus acidilactici | EU147316 | 99 | 345 |
805 | 25 | 2 | Whey starter | Pediococcus acidilactici | EU147316 | 100 | 345 |
Type LMG 6897 | Lactococcus lactis subsp. cremoris | 318 (272) | |||||
Type LMG 6896 | Streptococcus thermophilus | 319 (273) | |||||
Type LMG 6890 | Lactococcus lactis subsp. lactis | 318 (272, 291) | |||||
Type LMG 11423 | Enterococcus faecium | 328, 331 (284, 301, 303) | |||||
Type LMG 7937 | Enterococcus faecalis | 329 (283, 299) | |||||
Type LMG 6901 | Lactobacillus delbrueckii subsp. bulgaricus | 330 | |||||
Type LMG 11457 | Lactobacillus parabuchneri | 345 (255, 299) |
Quantity of strains isolated for this RAPD-PCR cluster or branch.
Determined by 16S-rRNA BLAST.
GenBank.
The fragment length is reported with an approximation of ±1 bp. The lengths of the secondary peaks are reported in parentheses.