TABLE 2.
Quantitation of total intracellular c-di-GMP for V. cholerae strains
| Genotypea | % c-di-GMP (±SD)b,d | Fold change in c-di-GMP (P value)c,d |
|---|---|---|
| WT | 0.069 (0.01) | 1 |
| ΔvieA | 0.56 (0.10) | 8.1 (<0.0004) |
| vieA(E170A) | 0.96 (0.29) | 14 (<0.005) |
| vieA ΔHTH | 0.24 (0.01) | 3.3 (<0.0001) |
| ΔvieS | 0.75 (0.10) | 11 (<0.0001) |
a
Strains were grown to mid-exponential growth phase in MOPS minimal medium containing radiolabeled orthophosphate. WT, wild type.
b
Two-dimensional thin-layer chromatography quantitation of radiolabeled guanine nucleotides was carried out to determine levels of c-di-GMP.
c
c-di-GMP levels are expressed as n-fold increases relative to the wild-type level of c-di-GMP normalized to total cell guanine nucleotide levels.
d
Student's t test was used to determine statistical significance.