TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 (Φ80dlacZΔM15) hsdR17 recA1 gyrA96 thi-1relA1 | Lab strain |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| ECAbrB | F−ompT hsdSB(rB− mB−) gal dcm (DE3)::pPHOP | This work |
| Bacillus amyloliquefaciens strain | ||
| FZB45 | wt | FZB Berlin |
| Bacillus subtilis | ||
| MF1 | trpC2 pheA1 rpoCHis6 Neor | M. Fujita |
| 168 | trpC2 | Laboratory sock |
| 1S13 | trpC2 spo0A1 | BGSC |
| OM61 | trpC2 amy::lacZ-phyC (495 bp) cat (Cmr) | This work |
| OM61Km | trpC2 amy::lacZ-phyC (495 bp) cat:aphA3 (Kmr) | This work |
| OM71 | trpC2 amy::lacZ-phyC (316 bp) cat | This work |
| OM545 | trpC2 amy::lacZ-phyC (355 bp) cat | This work |
| OM57 | trpC2 amy::lacZ-phyC (176 bp)Cmr | This work |
| OM64 | trpC2 spo0A1 amy::lacZ-phyC (495 bp) cat | This work |
| OM64Km | trpC2 spo0A1 amy::lacZ-phyC (495 bp) cat:aphA3 | This work |
| OM613 | trpC2 abrB::Cmr amy::lacZ-phyC (495 bp) cat:aphA3 | This work |
| OM612 | trpC2 spo0A1 abrB::lacZ-phyC (495 bp) cat | This work |
| OM74 | trpC2 spo0A1 amy::lacZ-phyC (316 bp) cat | This work |
| OM45 | trpC2 spo0A1 amy::lacZ-phyC (355 bp) cat | This work |
| OM457 | trpC2 spo0A1 amy::lacZ-phyC (176 bp) cat | This work |
| EB21 | trpC2 amy::lacZ-phyCabrB2 (495 bp) cat | This work |
| EB24 | trpC2 spo0A1 amy::lacZ-phyCabrB2 (495 bp) cat | This work |
| EB31 | trpC2 amy::lacZ-phyCabrB31 (495 bp) cat | This work |
| EB34 | trpC2 spo0A1 amy::lacZ-phyCabrB (495 bp) cat | This work |
| EB41 | trpC2 amy::lacZ-phyCabr312 (495 bp) cat | This work |
| EB44 | trpC2 spo0A1 amy::lacZ-phyCabrB32 (495 bp) cat | This work |
| Plasmids | ||
| pDG268 | Integrative vector amy::lacZ cat bla (Apr) | C. Antoniewski |
| pOM6 | pDG268/phyC from −287 (Om01) to +208 (Om09) → 495-bp insert | This work |
| pOM7 | pDG268/phyC from −287 (Om01) to +29 (Om14) → 316-bp insert | This work |
| pCUT5 | pDG268/phyC from −147 (Cut5) to +208 (Om09) → 355-bp insert | This work |
| pCUT57 | pDG268/phyC from −147 (Cut5) to +29 (Om14) → 176-bp insert | This work |
| pEB2 | pOM6, substitution of AbrB site 1 (eb2) | This work |
| pEB3 | pOM6, substitution of AbrB site 1 (eb3) | This work |
| pEB4 | pOM6, substitution of AbrB site 1 (eb4) | This work |
| pEB5 | pOM6, substitution of AbrB site 2 (eb5) | This work |
| pEB6 | pOM6, substitution of AbrB site 2 (eb6) | This work |
| pEB23 | pOM6, double substitution of AbrB site 1 (eb2 and eb3) | This work |
| pEB36 | pOM6, double substitution of AbrB sites 1 (eb3) and 2 (eb6) | This work |
| pET15b | Expression vector, T7lac lacI bla | Novagen |
| pABRB | pET15b/abrB of B. subtilis → 354-bp insert | This work NdeI/XhoI site |
| pABRBQ82K | pET15b/abrB of B. subtilis → 354-bp insert, substitution C:A (−245*) | This work NdeI/XhoI site |
| pPHOP | pET15b/phoP of B. subtilis → 728-bp insert | 11 |
| pPHOR231 | pET28b(+)/C-terminal domain of phoR of B. subtilis → 1,051-bp insert | 11 |
| pECE73 | Cmr::Kmr | M. Steinmetz/R. Rich |
a
*, position of the substitution of abrB is indicated relative to translation start. Neor; neomycin resistance.