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Effects of insulin and GTPγS on cell surface TfR levels. 3T3-L1 adipocytes were permeabilized with α-toxin as outlined in MATERIALS AND METHODS and incubated for 5 min before addition of insulin (1 μM) or GTPγS (100 μM) for 15 min at 37°C. Cells were then rapidly transferred to ice-cold buffer for assay of cell surface TfR levels. Shown are data from a representative experiment; each point is the mean of triplicate determinations (± SEM).
