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. 1981 Oct;40(1):118–125. doi: 10.1128/jvi.40.1.118-125.1981

Restriction map of the single-stranded DNA genome of Kilham rat virus strains 171, a nondefective parvovirus.

P T Banerjee, R Rothrock, S Mitra
PMCID: PMC256601  PMID: 6270368

Abstract

We constructed a physical map of Kilham rat virus strains 171 DNA by analyzing the sizes and locations of restriction endonuclease-generated fragments of the replicative-form viral DNA synthesized in vitro. BglI, KpnI, BamHI, SmaI, XhoI, and XorII did not appear to have any cleavage sites, whereas 11 other enzymes cleaved the genome at one to eight sites, and AluI generated more than 12 distinct fragments. The 30 restriction sites that were mapped were distributed randomly in the viral genome. A comparison of the restriction fragments of in vivo- and in vitro-replicated replicative-form DNAs showed that these DNAs were identical except in the size or configuration of the terminal fragments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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