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. 2008 Jul 25;190(19):6493–6500. doi: 10.1128/JB.00790-08

FIG. 1.

FIG. 1.

SDS-PAGE of the samples obtained during purification of PCMH and content of covalently bound FAD. (A) Shown is a 12.5% polyacrylamide gel. Lanes: M, molecular mass standard; 1, membrane fraction after ultracentrifugation; 2, washed membrane fraction; 3, LDAO-treated membrane; 4, supernatant after LDAO treatment; 5, NaCl-treated membrane; 6, supernatant after NaCl treatment; 7, gel filtration pool; 8, pool after hydrophobic interaction chromatography. (B) Shown is an 8% polyacrylamide gel of purified PCMH. Lanes: M, molecular mass standard; 9, fluorescence of 1 and 1.5 μg purified PCMH (excitation at 488 nm, detection at 520 nm); 10, Coomassie blue staining of the same gel. (C) Shown is a 16.5% polyacrylamide gel of purified PCMH. Lanes: M, molecular mass standard; 11, purified PCMH. For lanes M, masses in kDa are indicated to the left.