FIG. 3.
Inhibition of p-cresol oxidation by p-hydroxybenzyl alcohol. The Km values for p-cresol were determined in the presence of 0, 70, and 200 μM p-hydroxybenzyl alcohol by varying the p-cresol concentration in the presence of constant concentrations of p-hydroxybenzyl alcohol; the initial rates were was extrapolated from the regression curves for 1.7 (✸), 5.2 (•), 10.4 (⧫), 20.9 (▾), 40.0 (▴), and 120 (▪) μM p-cresol. (Left) Cornish-Bowden plot (10); (right) Dixon plot (4). Values for Kic (18 ± 9 μM) and Kiu (235 ± 20 μM) were estimated from the intersection points of the regression lines.