TABLE 2.
Spectral band | Absorption coefficient (M−1 cm−1)
|
||
---|---|---|---|
Oxidized | Reduced | Difference | |
α (552 nm) | 20,800 | 42,200 | 21,400 |
β (522 nm) | 26,700 | 30,600 | 3,900 |
Soret (410/416 nm) | 182,100 (410 nm) | 206,800 (416 nm) | 140,400a |
FAD (450 nm) | 48,100 | 24,700 | 23,400 |
The molar absorption coefficient for the Soret is calculated from the maximum (418 nm) and the minimum (406 nm) of the difference spectrum.