TABLE 3.

Molecular and kinetic properties of PCMH

Property	Value
Substrates (Km values [μM])	p-Cresol (1.7 ± 0.3); p-hydroxybenzyl alcohol (2.7 ± 0.7)
Products	p-Hydroxybenzyl alcohol; p-hydroxybenzaldehyde
Subunit composition	αα'β2; α, 57 kDa; α′, 59 kDa; β, 8.5 kDa
Native mol mass	124 ± 15 kDa
Cofactors	One covalently linked FAD, two heme c's
Absorption maxima	Reduced, 416, 523, and 552 nm; oxidized, 410 and 522 nm, shoulder at 450 nm
Specific activities (μmol min−1 mg−1)/turnover nos. (s−1)	2.2/4.8 (p-cresol); 0.2/0.48 (p-hydroxybenzyl alcohol)
kcat/Km (mol−1 s−1)	2.98 × 106 (p-cresol); 0.20 × 106 (p-hydroxybenzyl alcohol)
Inhibition constants for p-hydroxybenzyl alcohol	Kic = 18 ± 9 μM; Kiu = 235 ± 20 μM