TABLE 1.
Sequences of the primers and probes used in RT-PCR
| Organism assayed | RT-PCR primer or probe
|
|
|---|---|---|
| Name | Sequence (5′-3′)a | |
| S. apiospermum | Sap1 (forward primer) | 5′-CCATTGTGAACCTTACCTATG-3′ |
| Sap2 (reverse primer) | 5′-TCAAATCAGAACTGTAATCCG-3′ | |
| Sap-MB1 (probe) | FAM-CGCGATACGCCGCCGAGGCAACAGATCGCG-BHQ1 | |
| S. prolificans | Sp1 (forward primer) | 5′-AACCCTTTGTGAACCTTACC-3′ |
| Sp2 (reverse primer) | 5′-CGATGCCAGAACCAAGAG-3′ | |
| Sp-MB1 (probe) | FAM-CGCGATCCACCAAACTCTTGCATTTATAGCGGATTAGATCGCG-BHQ1 | |
a
Underlining indicates the PCR target sequence.