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Lane L contains the DNA ladder. Lanes 1 to 10 contain DNA extracts from bacteria isolated from patient samples at undiluted and 10−1 dilution. Lane 11 contains a positive control at a dilution of 10−6, and lane 12 contains the nontemplate control (ultrapure water [UPW]). The product demonstrated is an lpxO PCR amplicon of 110 bp in length. pos, positive.
