TABLE 1.
Summary of deployable laboratory results
Samplea | Typeb | Location in Australia, source | Culture resultc | lpxO PCR result |
---|---|---|---|---|
A | Clinical, blood | Central Province | B. pseudomallei | + |
B | Clinical, synovial fluid | Central Province | B. pseudomallei | + |
C | Clinical, blood | Uva Province | B. pseudomallei | + |
D | Clinical, blood | Colombo | Unidentified GNB | − |
E | Clinical, blood | Colombo | Unidentified GNB | − |
AA | Env, SS | Kandy area, rubber tree | NA | + |
AB | Env, SS | Kandy area, rubber tree | NA | − |
AC | Env, SS | Kandy area, banana palm | NA | + |
AD | Env, SS | Kandy area, rice terrace | NA | + |
AE | Env, SS | Kandy area, rice terrace | NA | − |
BA | Env, BPSA isolate | Kandy area | Unidentified GNB | − |
BB | Env, BPSA isolate | Kandy area | Unidentified GNB | − |
BC | Env, BPSA isolate | Kandy area | Unidentified GNB | − |
BD | Env, BPSA isolate | Kandy area | Unidentified GNB | − |
BE | Env, BPSA isolate | Kandy area | Unidentified GNB | − |
Serial samples A to E, clinical isolates; AA to AE, soil supernatants; BA to BE, preliminary isolates from soil supernatants on B. pseudomallei selective agar. All serial samplings were repeated at least once.
Env, environmental; SS, soil supernatant; BPSA, B. pseudomallei selective agar.
The duration of laboratory deployment did not permit preliminary identification of soil bacteria by conventional phenotypic laboratory methods. The PCR protocol was therefore used as a rapid screening method for identification of oxidase-positive, gentamicin-resistant, gram-negative bacilli. GNB, gram-negative bacillus; NA, not applicable.