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. 2008 Aug 20;46(10):3479–3481. doi: 10.1128/JCM.01254-08

TABLE 1.

Summary of deployable laboratory results

Samplea Typeb Location in Australia, source Culture resultc lpxO PCR result
A Clinical, blood Central Province B. pseudomallei +
B Clinical, synovial fluid Central Province B. pseudomallei +
C Clinical, blood Uva Province B. pseudomallei +
D Clinical, blood Colombo Unidentified GNB
E Clinical, blood Colombo Unidentified GNB
AA Env, SS Kandy area, rubber tree NA +
AB Env, SS Kandy area, rubber tree NA
AC Env, SS Kandy area, banana palm NA +
AD Env, SS Kandy area, rice terrace NA +
AE Env, SS Kandy area, rice terrace NA
BA Env, BPSA isolate Kandy area Unidentified GNB
BB Env, BPSA isolate Kandy area Unidentified GNB
BC Env, BPSA isolate Kandy area Unidentified GNB
BD Env, BPSA isolate Kandy area Unidentified GNB
BE Env, BPSA isolate Kandy area Unidentified GNB
a

Serial samples A to E, clinical isolates; AA to AE, soil supernatants; BA to BE, preliminary isolates from soil supernatants on B. pseudomallei selective agar. All serial samplings were repeated at least once.

b

Env, environmental; SS, soil supernatant; BPSA, B. pseudomallei selective agar.

c

The duration of laboratory deployment did not permit preliminary identification of soil bacteria by conventional phenotypic laboratory methods. The PCR protocol was therefore used as a rapid screening method for identification of oxidase-positive, gentamicin-resistant, gram-negative bacilli. GNB, gram-negative bacillus; NA, not applicable.