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Carboxyl-terminal two residues function as an anterograde transport signal. Mutants were made as indicated in Figure 1. Pulse–chase experiments were done, and transport rate constants were calculated as described for Figures 2 and 3. (A) Rate constants of transport to the vacuole. (B) Rate constants of ER to cis-Golgi transport. Bars, mean values; error bars, SD. The numbers of experiments are shown on top of the bars.
